Technical Data
Syntaxin 6 (Syntaxin-6, STX6, HGNC:11441)
Syntaxin 6 is a 35kD type II integral membrane protein with two coiled coil domains and a carboxyl terminal membrane anchor. Immunoelectron microscopy studies show that syntaxin 6 is primarily localized to the trans Golgi network (TGN), and is partially co localized with AP 1 on clathrin coated membranes. During secretory granule maturation, syntaxin 6 and MPR ligand complexes are postulated to be transferred from immature granules to endosomes by clathrin coated vesicles, as evidenced by immunoelectron microscopy. These results suggest that syntaxin 6 mediates a TGN trafficking targeting endosome.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.3-1ug/ml, Approx 30kDa band observed in Human Lymph Node lysates (calculated MW of 29.2kDa according to NP_005810.1).
Optimal dilutions to be determined by the researcher.

Positive Control: Human Lymph Node

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ug-20CBlue IceHumanGoat
C-QALAERKNRQAinternal regionNP_005810.1. Homology: Mouse, Rat, Dog
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
Species Crossreactivity: Human. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Murray RZ, Wylie FG, Khromykh T, Hume DA, Stow JL. Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha.J Biol Chem. 2005 Mar 18;280(11):10478-83. Epub 2005