Technical Data
S9205-45
Syntaxin 13 (Syntaxin-13, SYX 13, SYX13, CG11278, CT31479, DmSyx13, Dm-Syx13, ff16, ff16 (69D1-70A2), l(3)01470)
Description:
Syntaxins are type II integral membrane proteins localized to membranes that regulate vesicle transport, protein maturation, and exocytosis. Syntaxin 1A resides in the nerve terminals of sensory neurons and nerve fibers reaching small blood vessels. Syntaxin 2 localizes to the apical plasma membrane and regulates epithelial-mesenchymal interactions. Syntaxin 6 is primarily localized to the trans-Golgi network, and partially co-localizes with AP-1 on clathrin-coated membranes. Syntaxin 13 is ubiquitously expressed and localizes to the tubular early and recycling endosomes, as well as endosomal vacuoles.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (Colorimetric): 1:1000
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Positive Controls:
Rat Brain Tissue Extract and Mouse Brain Tissue Extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG4E212Affinity Purified
SizeStorageShippingSourceHost
25ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant cytoplasmic domain of rat syntaxin 13.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes rat Syntaxin 13. Detects an ~38kD protein, corresponding to the apparent molcular mass of syntaxin 13. Species Crossreactivity: mouse, canine and hamster
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Advani, R.J., et al. (1998) J. Biol. Chem. 273: 10317-10324. 2. Prekeris, R., Klumperman, J., Chen, Y.A. and Scheller, R.H. (1998) J. Cell Biol. 143:
957-971. 3. Huang, L; Kuo, YM; Gitschier, J; (1999) Nat Genet 23(3): 329-332.