Technical Data
S9205-80B
Syntaxin-binding Protein 1 (STXBP1, FLJ37475, MUNC18-1, NSEC1, N-Sec1, Protein unc-18 Homolog 1, Unc18-1, Protein unc-18 Homolog A, Unc-18A, p67, RBSEC1, UNC18, UNC18A)
Description:
Munc-18, otherwise known as STXBP1, is a syntaxin-binding, pre-synaptic protein and member of the STXBP/UNC-18/SEC1 family, expressed in the brain and spinal cord, and at significantly high levels in neuronal axons. Munc-18 is a vital component of the synaptic vesicle fusion protein complex, playing a critical role in calcium-dependent neurotransmitter release, and in the regulation of the exocytosis of neurons and neuroendocrine cells. Studies have shown that isoform a of Munc-18 (Munc 18-1/Munc18a), acts as a regulator of insulin secretion from pancreatic beta cells.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CSRVSFEDQAPTME, from the C-terminus of the protein sequence according to NP_003156.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human STXBP1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Swanson DA, Steel JM, Valle D. Identification and characterization of the human ortholog of rat STXBP1, a protein implicated in vesicle trafficking and neurotransmitter release. Genomics. 1998 Mar 15;48(3):373-6. PMID: 9545644