Technical Data
S9500-01B
Synuclein, alpha
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5,000-1:10,000 detects a-Synuclein recombinant protein and a-Synuclein in Alzheimer’s diseased human whole brain lysates. a-Synuclein recombinant protein was resolved by electrophoresis, transferred to nitrocellulose and probed with anti--Synuclein (1:10,000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Immunohistochemistry: This antibody has been reported by an independent laboratory to detect a-Synuclein in human midbrain tissue sections (1).

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
Mab4H375Ascites
SizeStorageShippingSourceHost
200ul-20°CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
HPLC-purified full length recombinant human a-Synuclein.
Purity:
Ascites
Form
Supplied as a liquid with 0.05% sodium azide.
Specificity:
Recognizes a-Synuclein, Mr ~14kD. Species Crossreactivity: Human and zebra finch. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Application Reference:
1. Giasson, B.I., et al., J. Neurosci. Res. 59: 528-533, 2000.
General Reference:
2. Tu, P., et al., Ann. Neurol. 44: 415-422, 1998.