Technical Data
S9500-03T
Synuclein, alpha (SNCA, Alpha Synuclein, MGC110988, Non-A beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, PARK1, PARK4, Parkinson Disease Familial 1, PD1)
Description:
The implication of alpha synuclein in neurodegenerative diseases started after the identification of non A beta component of amyloid (NAC peptide) in Alzheimer's disease (AD) brains. There is now a general group of synucleinopathies which implicates alpha synuclein in Lewy body syndromes, Parkinson' disease and Alzheimer's disease. Certain missense mutations in the alpha synuclein gene (A53T, A30P) have been linked to the familial Parkinson's disease (PD). Alpha Synuclein is the major component of Lewy bodies and Lewy neuritis in sporadic PD, dementia with Lewy Bodies and Lewy Body variant of AD. Lewy bodies are composed of truncated and phosphorylated intermediate neurofilament proteins, alpha synuclein, ubiquitin and associated enzymes. The synuclein phosphoproteins (15-20kD) are small highly conserved proteins in vertebrates. The synuclein family includes alpha and beta synucleins and loosely related gamma synuclein and synoretin. The expression is abundant in neurons and typically is localized at presynaptic terminals. Antibodies to alpha synuclein provide a specific method to detect Lewy bodies and associated pathological mechanism of PD and AD.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:100 for 10 min at RT. No special pretreatment is required for staining formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Posterior cingulated gyrus

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to aa111-131 from near the C-terminal of human alpha Synuclein (Accession #NP_000336.1). Cellular Localization: Cytoplasmic, nuclear. Membrane bound in dopaminergic neurons.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Specificity:
Recognizes human alpha Synuclein. Does not crossreact with beta synuclein. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.