		Technical Data
S9500-03U	Synuclein, alpha (Alpha-synuclein, SNCA, MGC110988, Non-A beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, PARK1, PARK4, PD1)

Description:
Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson’s disease (PD) and other neurodegenerative disorders. Mutation of the alpha-synuclein gene is associated with familial forms of PD. Recent evidence suggests that phosphorylation of alpha-synuclein at ser129 plays a role in the formation of inclusions in PD.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
K562 cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic non-phosphopeptide corresponding to human a-Synuclein around the phosphorylation site of tyrosine 125 (E-A-YP-E-M ).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes human a-Synuclein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Masliah, E. et al. Neuron. 46:857-868 (2005). 2. Smith, WW. et al. J. Neurosci. 25:5544-5552 (2005).