Technical Data
S9500-03U
Synuclein, alpha (Alpha-synuclein, SNCA, MGC110988, Non-A beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, PARK1, PARK4, PD1)
Description:
Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson’s disease (PD) and other neurodegenerative disorders. Mutation of the alpha-synuclein gene is associated with familial forms of PD. Recent evidence suggests that phosphorylation of alpha-synuclein at ser129 plays a role in the formation of inclusions in PD.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
K562 cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human a-Synuclein around the phosphorylation site of tyrosine 125 (E-A-YP-E-M ).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human a-Synuclein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Masliah, E. et al. Neuron. 46:857-868 (2005). 2. Smith, WW. et al. J. Neurosci. 25:5544-5552 (2005).