Technical Data
S9500-06B
Synuclein, alpha (Alpha-Synuclein, SNCA, Non-A beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, PARK1, PARK4, PD1)
Description:
Alpha-synuclein is present in brain tissue and studies implicate the protein in Lewy body formation and Parkinson’s disease. This antibody will help to detect alpha-synuclein expression.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 1:20-1:40 for 1hr at 25°C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Posterior cingulated gyrus, lewy body and neurophil staining.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kKM51Supernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Recombinant protein representing most of the full-length alpha synuclein molecule.
Purity:
Supernatant
Form
Supplied as a liquid in 15mM sodium azide.
Specificity:
Recognizes human Alpha Synuclein. Does not recognize beta synuclein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Baba M, Nakajo S, Tu P-H, et al.. Aggregation of a-synuclein in Lewy bodies of sporadic Parkinson’s disease and dementia with Lewy bodies. American Journal of Pathology. 152(4): 879-884 (1998). 2. Polymeropoulos M H, Lavedan C, Leroy E, et al.. Mutation in the a-synuclein gene identified in families with Parkinson’s disease. Science. 276:2045- 2047 (1997). 3. Spillantini M G, Schmidt M L, Lee V M-Y, et al.. a-synuclein in Lewy bodies. Nature. 388:839-840 (1997). 4. Iwai A, Masliah E, Sundsmo M P, et al.. The synaptic protein NACP is abnormally expressed during the progression of Alzheimer’s disease. Brain Research. 720:230-234 (1996). 5. Masliah E, Iwai A, Mallory M, et al.. Altered presynaptic protein NACP is associated with plaque formation and neurodegeneration in Alzheimer’s dis- ease. American Journal of Pathology. 148(1): 201-210 (1996). 6. Weinreb P H, Zhen W, Poon A W, et al.. NACP, a protein implicated in Alzheimer’s disease and learning, is natively unfolded. Biochemistry. 35(43):13709-13715 (1996). 7. Xia Y, de Silva H A R, Rosi B L, et al.. Genetic studies in Alzheimer’s disease with an NACP/a-synuclein polymorphism. Annals of Neurology. 40(2): 207-215 (1996).