		Technical Data
S9500-06B	Synuclein, alpha (Alpha-Synuclein, SNCA, Non-A beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, PARK1, PARK4, PD1)

Description:
Alpha-synuclein is present in brain tissue and studies implicate the protein in Lewy body formation and Parkinson’s disease. This antibody will help to detect alpha-synuclein expression.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 1:20-1:40 for 1hr at 25°C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Posterior cingulated gyrus, lewy body and neurophil staining.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	KM51	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice		Mouse

Concentration:

Not determined

Immunogen:

Recombinant protein representing most of the full-length alpha synuclein molecule.

Purity:

Supernatant

Form

Supplied as a liquid in 15mM sodium azide.

Specificity:

Recognizes human Alpha Synuclein. Does not recognize beta synuclein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Baba M, Nakajo S, Tu P-H, et al.. Aggregation of a-synuclein in Lewy bodies of sporadic Parkinson’s disease and dementia with Lewy bodies. American Journal of Pathology. 152(4): 879-884 (1998). 2. Polymeropoulos M H, Lavedan C, Leroy E, et al.. Mutation in the a-synuclein gene identified in families with Parkinson’s disease. Science. 276:2045- 2047 (1997). 3. Spillantini M G, Schmidt M L, Lee V M-Y, et al.. a-synuclein in Lewy bodies. Nature. 388:839-840 (1997). 4. Iwai A, Masliah E, Sundsmo M P, et al.. The synaptic protein NACP is abnormally expressed during the progression of Alzheimer’s disease. Brain Research. 720:230-234 (1996). 5. Masliah E, Iwai A, Mallory M, et al.. Altered presynaptic protein NACP is associated with plaque formation and neurodegeneration in Alzheimer’s dis- ease. American Journal of Pathology. 148(1): 201-210 (1996). 6. Weinreb P H, Zhen W, Poon A W, et al.. NACP, a protein implicated in Alzheimer’s disease and learning, is natively unfolded. Biochemistry. 35(43):13709-13715 (1996). 7. Xia Y, de Silva H A R, Rosi B L, et al.. Genetic studies in Alzheimer’s disease with an NACP/a-synuclein polymorphism. Annals of Neurology. 40(2): 207-215 (1996).