Technical Data
S9500-08
Synuclein, alpha
Description:
This antibody recognizes a-synuclein, which belongs to a family of small cytoplasmic proteins expressed predominantly in neurons. The epitope maps to amino acid residues 121-125 of human a-synuclein. a-synuclein may be involved in neuronal plasticity and could act as a molecular chaperone that mediates the transformation of soluble AB into insoluble amyloid. The protein is a major component of Lewy bodies, the pathological hallmark of Parkinson’s disease, and is also observed in senile plaques of Alzheimer’s disease patients. Human a-synuclein appears to be phosphorylated at two major sites, serine 129 and serine 87, and phosphorylation may play a role in the functional regulation of the protein.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1.0ug/ml
Immunohistochemistry: formalin-fixed paraffin sections
Optimal dilutions to be determined by the researcher.

Recommended Positive Control: Human SHSY-5Y cells.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12B2.99Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant human a-synuclein.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
Specificity:
Recognizes alpha-synuclein (19kD). Species Crossreactivity: Human. Does not crossreact with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bruening, W., et al. (2000) Synucleins are expressed in the majority of breast and ovarian carcinomas and in preneoplastic lesions of the ovary. Cancer 88(9):2154-2163. 2. Giasson, B.I., et al. (2000) A panel of epitope-specific antibodies detects protein domains distributed throughout human -synuclein in Lewy bodies of Parkinson's disease. J. Neurosci. Res. 59(4):528-533 .3. Giasson, B.I. (2000) Oxidative damage linked to neurodegeneration by selective alpha-synuclein nitration in synucleinopathy lesions. Science 290:985-989. 4. McLean, P. J., et al. (2000) Membrane association and protein conformation of -synuclein in intact neurons. J. Biol. Chem. 275(12):8812-8816. 5. Okochi, M., et al. (2000) Constitutive phosphorylation of the Parkinson’s disease associated -synuclein. J. Biol. Chem. 275(1):390-397.