Technical Data
S9500-11
Synuclein, alpha
Description:
Synnuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimer’s (AD), Parkinson’s (PD) and breast cancer. In AD, a peptide derived from alpha-synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha-synuclein accumulates in Lewy bodies. An allele of alpha-synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds.

Applications:
Suitable for use in Western Blot, Immnoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (formalin/paraffin): 1:200 for 30 min at RT
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG13F344Affinity Purified
SizeStorageShippingSourceHost
250ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
0.1mg/ml
Immunogen:
Human recombinant alpha-synuclein. Cellular Localization: Neuronal cytosol and presynaptic terminals MW of Antigen: ~14kD
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See S9500-11X
Specificity:
Recognizes Human recombinant alpha-synuclein. Species Crossreactivity: Highly specific for alpha-synuclein and does not react with other synuclein subtypes. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1 Giasson B I, et al. (2000) J. Neurosci Res 59: 528–533.