Technical Data
S9500-11X
Synuclein, alpha (BSA & Azide Free)
Description:
Synuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimer’s (AD), Parkinson’s (PD) and breast cancer. In AD, a peptide derived from alpha-synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha-synuclein accumulates in Lewy bodies. An allele of alpha-synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:400 for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13F344Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human recombinant alpha-synuclein. MW: ~14kD. Cellular Localization: Neuronal cytosol and presynaptic terminals.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See S9500-11.
Specificity:
Highly specific for human alpha-synuclein. Does not react with beta or gamma synuclein. Species Crossreactivity: Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Giasson, B.I., et al., J. Neurosci Res 59: 528–533 (2000).