Recommended Secondary Reagents: Donkey anti-SheepGoat IgG: HRP
Recommended Positive Control Tissue: Brain, Spinal Cord.
Immunohistochemistry: Paraffin. 1:300
ELISA: 1:1000 vs immunizing peptide
Optimal working dilutions to be determined by researcher.
Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
| Not determined|
|Synthetic peptide corresponding to amino acids 116-131 of the C-terminus of a synuclein.|
|Serum. Supplied as a liquid with 0.1% sodium azide.|
|Recognizes human alpha-synuclein. The alpha-synuclein gene, located on chromosome 4, has been linked to early onset autosomal dominant Parkinson's disease. The protein is abundant in the nervous system and is known to be the precursor of the non-beta amyloid component protein (NACP) of Alzheimer's disease. Alpha-synuclein has also been found to be associated with other neurodegenerative diseases such as multiple system atrophy and dementia with Lewy's bodies.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Spillantini, M.G. et al. (1997). a-synuclein in Lewy bodies. Nature 388: 839-840.2. Gai, W.P. et al. (1997). Multiple-system atrophy: a new alpha-synuclein disease. Lancet 352: 547-548.|