Technical Data
S9500-21A
Synuclein, alpha, CT (SNCA, 127750, P37840, MGC110988, NACP, PARK1, PARK4, non-A beta Component of AD Amyloid, NACP, Parkinson Disease Familial 1, PD1)
Description:
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. An early step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synuclein is identical with non-Ab component (NACP) of AD. The 134aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold.

Applications:
Suitable for use in ELISA and Western Blot.
Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide: S9500-21P

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 15aa located near the C-terminus of human synuclein-alpha.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human a-Synuclein CT. No significant homology is seen with beta-synuclein or with other known proteins. Species sequence homology: Rat, mouse and chicken: 86%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jakes et al (1994) FEBS Lett. 345, 27-32. 2. Ueda K et al (1993) PNAS 90, 11282-11286. 3. Maroteaux L. et al (1988) J Neurosci. 8, 2804-2815. 4. Maroteaux L et al (1991) Mol. Brain Res. 11, 335-343.