Technical Data
S9500-21C
Synuclein, alpha (SNCA, 127750, P37840, MGC110988, NACP, PARK1, PARK4, non-A beta Component of AD Amyloid, NACP, Parkinson Disease Familial 1, PD1)
Description:
a-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. a-synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Mutations of the a-synuclein gene have been linked to Parkinson disease (1).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochmistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:200 Requires citrite HIER.
Immunofluorescence (IF-F): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10E50Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide surrounding Glu105 of mouse a-synuclein protein.
Purity:
Supernatant
Form
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes mouse alpha Synuclein at 18kD. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.