		Technical Data
S9500-21C	Synuclein, alpha (SNCA, 127750, P37840, MGC110988, NACP, PARK1, PARK4, non-A beta Component of AD Amyloid, NACP, Parkinson Disease Familial 1, PD1)

Description:
a-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. a-synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Mutations of the a-synuclein gene have been linked to Parkinson disease (1).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochmistry, and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:200
Immunofluorescence (IF-F): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	10E50	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide surrounding Glu105 of mouse a-synuclein protein.

Purity:

Supernatant

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes mouse alpha Synuclein at 18kD. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.