Technical Data
S9500-23
Synuclein, alpha, Nitrated
Description:
Alp[ha-synuclein belongs to the synuclein family, which also includes b- and g-synuclein, and is predominantly expressed in neurons, concentrated at synaptic terminals. Synucleins may be involved in the regulation of dopamine release and transport and the modulation of synaptic vesicle function. a-synuclein is a small, presynaptic protein that is the major non-amyloid component of the pathological inclusions characteristic of a wide range of neurodegenerative disorders, collectively known as synucleinopathies. Synucleinopathies include Parkinson’s disease (PD), Lewy body variant of Alzheimer’s disease (LBVAD), dementia with Lewy bodies (DLB), neurodegeneration with brain iron accumulation (NBIA-1), and multiple system atrophy (MSA). Although alpha-synuclein is a soluble protein, it polymerizes into filaments in vitro under a variety of conditions, lending more support to the finding that it is the major building block of the filaments that form Lewy bodies (LBs). alpha-synuclein is known to reduce the fibrillization of the microtubule-associated protein, tau, and to bind various targets such as 14-3-3, protein kinase C, synphilin-1, Elk, and Tat-binding protein 1.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IC): 1-5ug/ml mouse
ELISA: 0.1-1.0ug/ml immunogen
Western Blot: 1-3ug/ml Band at ~18kD
Immunohistochemistry (Paraffin): 1-5ug human
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG4H316Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
0.5mg/ml
Immunogen:
Recombinant full-length oxidized human alpha-Synuclein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the nitrated/oxidized and non-nitrated forms of human a-Synuclein protein. Cross-reactivity with b- and g-synucleins has not been observed in testing with bacterially-expressed and purified recombinant proteins. Species sequence homology: mouse and rat: 95%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Duda J et al. Ann Neurol 52:205-210,2002. 2. George JM et al. Neuron 15:361-372,1995. 3. Giasson BI, et al. J Biol Chem 274(12):7619-7622,1999. 4. Giasson B et al. Neuron 34: 521-533,2002. 5. Ahn,BH et al. J Biol Chem 277:12334-1242,2002.