Technical Data
S9500-40
Synaptophysin
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneths cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 5-10ug/ml for 2 hours at RT.
Immunohistochemistry (formalin/paraffin): 5-10ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined bythe researcher.

Positive Control:
IMR-5 cells. Pancreas or Pheochromocytoma or GI tumor.

MW of Antigen: 38kD

Epitope: C-terminal

Cellular Localization: Cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide from C-terminus of human synaptophysin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See S9500-40X.
Specificity:
Reacts with neuroendocrine neoplasms of neural as well as epithelial types. In combination with anti-chromogranin A and anti-NSE, very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms. Species Crossreactivity: Human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Danielyan, L. et al., (2009) Cell Death Differ. 16:1599-1614. 1. Blumenfeld W; et al (1996) Archives of Pathology and Laboratory Medicine. 120(5):47881. 2. Dottorini ME, et al. (1996) Cancer, 77(8):155665.