Technical Data
Synaptophysin (SYP, Major Synaptic Vesicle Protein p38, MRXSYP)
Synaptophysin is found in neuronal presynaptic vesicles in brain, spinal cord, retina and similar vesicles of the adrenal medulla, and in neuromuscular junctions. It seems to play a role in synaptic vesicle formation and exocytosis. This antibody will help identify synapto- physin in normal and uncharacterized tissues.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Immunohistochemistry: 1:100-1:200 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: IMR-32 cell line
Immunohistochemistry: Thyroid. Cytoplasmic staining pattern.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20°CBlue IceMouse
Not determined
Synthetic peptide representing a region close to the C-terminal end of the synaptophysin molecule.
Supplied as a liquid in 15mM sodium azide.
Recognizes Synaptophysin. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kennedy W R, Wendelschafer-Crabb G and Brelje T C. Innervation and vasculature of human sweat glands: an immunohistochemistry-laser scanning confocal fluorescence microscopy study. The Journal of Neuroscience. 14(11):6825-6833 (1994). 2.Redecker P, Jörns A, Jahn R, et al.. Synaptophysin immunoreactivity in the mammalian endocrine pancreas. Cell Tissue Research. 264:461-467 (1991). 3.Stefaneanu L, Ryan N and Kovacs K. Immunocytochemical localization of synaptophysin in human hypophyses and pituitary adenomas. Arch Pathol Lab Med. 112:801-804 (1988). 4.Gould V E, Wiedenmann B, Lee I, et al.. Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am J Pathol. 126(2):243-257 (1987). 5.Gould V E, Lee I, Wiedenmann B, et al.. Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms. Human Pathology. 17(10):979-983 (1986). 6.Wiedenmann B, Franke W W, Kuhn C, et al.. Synaptophysin: a marker protein for neuroendocrine cells and neoplasms. Proc Natl Acad Sci USA. 83:3500-3504 (1986). 7.Wiedenmann B and Franke W W. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell. 41:1017-1028 (1985).