Synaptophysin is found in neuronal presynaptic vesicles in brain, spinal cord, retina and similar vesicles of the adrenal medulla, and in neuromuscular junctions. It seems to play a role in synaptic vesicle formation and exocytosis. This antibody will help identify synapto- physin in normal and uncharacterized tissues.
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:500
Immunohistochemistry: 1:100-1:200 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.
Western Blot: IMR-32 cell line
Immunohistochemistry: Thyroid. Cytoplasmic staining pattern.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Kennedy W R, Wendelschafer-Crabb G and Brelje T C. Innervation and vasculature of human sweat glands: an immunohistochemistry-laser scanning confocal fluorescence microscopy study. The Journal of Neuroscience. 14(11):6825-6833 (1994). 2.Redecker P, Jörns A, Jahn R, et al.. Synaptophysin immunoreactivity in the mammalian endocrine pancreas. Cell Tissue Research. 264:461-467 (1991). 3.Stefaneanu L, Ryan N and Kovacs K. Immunocytochemical localization of synaptophysin in human hypophyses and pituitary adenomas. Arch Pathol Lab Med. 112:801-804 (1988). 4.Gould V E, Wiedenmann B, Lee I, et al.. Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am J Pathol. 126(2):243-257 (1987). 5.Gould V E, Lee I, Wiedenmann B, et al.. Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms. Human Pathology. 17(10):979-983 (1986). 6.Wiedenmann B, Franke W W, Kuhn C, et al.. Synaptophysin: a marker protein for neuroendocrine cells and neoplasms. Proc Natl Acad Sci USA. 83:3500-3504 (1986). 7.Wiedenmann B and Franke W W. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell. 41:1017-1028 (1985).|