Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Immunohistochemistry (Frozen): Neat
Immunohistochemistry (Paraffin): Requires microwave treatment prior to incubation.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not Determined|
|Isolated rat kidney glomeruli.|
|Supplied as a liquid, 0.01% sodium azide.|
|Recognizes rat synaptopodn. Reacts with differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation- dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. Species Crossreactivity: human, bovine, mouse, guinea pig and gerbil. Does not react with rabbit, frog, or chicken.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
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