Technical Data
T1017
TaqI
3000U
Molecular Biology Storage: -20CShipping: Blue Ice
5'-T^C G A-3'
3'-A G C^T-5'

Concentration:
10u/ul

Source:
Thermus aquaticus YT-1

Supplied wtih:
R1625-80 Restriction Enzyme Buffer for TaqI, 10X: Supplied as a liquid in
10mM Tris-HCl pH 8.0, 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Use as 1x. Incubate at 65C under paraffin oil in a capped vial. Incubation at 37C results in 10% activity.

R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37C.

Diluent Buffer:
10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periodsthe Storage Buffer should be used.

Storage Buffer:
10mM Tris-HCl pH 7.5, 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.

Compatible Ends:
Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, Ssil, XmiI

Unit Definition:
One unit is defined as the amount of TaqI to digest 1ug of lambda DNA dam- in 1 hour at 65C in 50ul of assay buffer.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Thermal Inactivation:
Enzyme is not inactivated by incubation at 80C for 20min.

Overdigestion Assay:
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA dam-x 16 hours) with TaqI.

Ligation/Recleavage Assay:
The ligation and recleavage assay was replace with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.

Labeled Oligonucleotide (LO) Assay:
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of TaqI for 4 hours.


Methylation Effects:
Dam: may overlap- blocked
Dcm, EcoKI, EcoBl: never overlaps-no effect
CpG: Completely overlaps- no effect

Stability during Prolonged Incubation:
A minimum of 0.3 units of TaqI is required for complete digestion of 1ug of lambda DNA in 16 hours at 65C.

Number of Recognition Sites in DNA:
Lambda: 121
PhiX174: 10
pBR322: 7
pUC57: 4
pUC18/19: 4
pTZ19R/U: 5
M13mp18/19: 12
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.