Technical Data
T1017
TaqI
3000u
Molecular Biology Storage: -20°CShipping: Blue Ice
5'-T^C G A-3'
3'-A G C^T-5'

Concentration:
10u/ul

Source:
Thermus aquaticus YT-1

Supplied wtih:
R1625-80 Restriction Enzyme Buffer for TaqI, 10X: Supplied as a liquid in
10mM Tris-HCl pH 8.0, 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Use as 1x. Incubate at 65°C under paraffin oil in a capped vial. Incubation at 37ēC results in 10% activity.

R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37ēC.

Diluent Buffer:
10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used.

Storage Buffer:
10mM Tris-HCl pH 7.5, 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.

Compatible Ends:
AciI, Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, Ssil, XmiI

Unit Definition:
One unit is defined as the amount of TaqI to digest 1ug of lambda DNA dam- in 1 hour at 65°C in 50ul of assay buffer.
 Thermal Inactivation:
Enzyme is not inactivated by incubation at 80°C for 20min.

Overdigestion Assay:
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA dam-x 16 hours) with TaqI.

Ligation/Recutting Assay:
After 50-fold overdigestion (3u/ug DNA x 17 hours) with TaqI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.2uM. More than 95% of these can be recut.

Labeled Oligonucleotide (LO) Assay:
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of TaqI for 4 hours.


Methylation Effects:
Dam: may overlap- blocked
Dcm, EcoKI, EcoBl: never overlaps-no effect
CpG: Completely overlaps- no effect

Stability during Prolonged Incubation:
A minimum of 0.3 units of TaqI is required for complete digestion of 1ug of lambda DNA in 16 hours at 65°C.

Number of Recognition Sites in DNA:
Lambda: 121
PhiX174: 10
M13mp18/19: 12
pBR322: 7
pUC18/19: 4
pUC57: 4
pTZ19R/U: 5
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.