Technical Data
T1029
Tau (Neurofibrillary Tangles Marker, Microtubule-associated Protein, MAP, Paired helical filament-tau, PHF-tau)
Description:
Tau proteins are important in the assembly of microtubules. In normal brain, Tau is localized within the axons of the neurons, but in some neuropathological lesions it accumulates within the body of the neuron. Tau proteins are thought to be abnormally phosphorylated in certain disease states. Tau has been immunohistochemically localized to neurofibrillary tangles of Alzheimer’s disease (AD) brains. Tau proteins promote the assembly of tubulin monomers into microtubules and stabilize microtubules. Alternate splicing of Tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of Tau.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Immunoprecipitation: 10ug/200-500ug of cell lysate
Immunohistochemistry (FFPE, frozen): 1-2ug/ml for 30-60 minutes at RT. For formalin-fixed paraffin embedded tissues, staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes prior to antibody incubation. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Observed to stain astrocytes.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Human T98G glioblastoma, SH-SY5Y cells or brain tissue.

Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12B2.100Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceBovineMouse
Concentration:
~0.5mg/ml
Immunogen:
Purified bovine microtubule-associated proteins.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes and is highly specific for non-phosphorylated and phosphorylated forms of bovine Tau proteins (45-68kD). Does not crossreact with other microtubule associated proteins (MAPs) or tubulin. The epitope is located in the middle region of Tau. Species Crossreactivity: human, sheep, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Rouzier, R. et al., (2005) PNAS 102:8315-8320. 2. Shao, Y.-Y., et al., (2010) Japanese J. of Clinical Oncology 40:286-293. 3. Bamias, A. et al., (2010) Cancer Chemotherapy and Pharmacology 65:1009-1021. 4. Bordeaux, J. et al., (2010) BioTechniques 48:197-209. 5. Baquero, M.T. et al., (2012) Cancer, DOI: 10.1002/cncr.27453. 1. Papasozomenos, S.C. & Shanavas, A., (2002) PNAS USA 99:1140-1145. 2. Rapoport, M., et al., (2002) PNAS USA 99:6364-6369. 3. Takahashi, S., et al., (2003) J. Biol. Chem. 278:10,506-10,515.