Technical Data
T1030-01P
Tau (Microtubule-associated Protein tau, MAPT, DDPAC, FTDP-17, FLJ31424, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, Neurofibrillary Tangle Protein, Paired Helical Filament-tau, PHF-tau, PPND)
Description:
Tau is the major microtubule-associated protein in neuronal axons that regulates microtubule stability, neurite outgrowth, and other microtubule -dependent functions. C-terminus of tau protein binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting its role as a linker protein between both. Six tau isoforms are produced in adult human brain by alternative mRNA splicing from a single gene. Filamentous tau protein deposits are the defining characteristic of various neurodegenerative diseases, many of which are frontotemporal dementias or movement disorders, such as Pick's disease, progressive supranuclear palsy, and corticobasal degeneration. human tau gene is mapped to chromosome 17q21.1.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat hippocampus tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human Tau around the phosphorylation site of threonine 212 (S-R-TP-P-S).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human Tau. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Michel, G. et al. Annals of the New York Academy of Sciences. 920:74-83 (2000). 2. Mukrasch, MD. et al. J. Biol. Chem. 282:12230-12239 (2007).