		Technical Data
T1030-01R	Tau (Microtubule-associated Protein tau, MAPT, DDPAC, FTDP-17, FLJ31424, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, Neurofibrillary Tangle Protein, Paired Helical Filament-tau, PHF-tau, PPND)

Description:
Tau is the major microtubule-associated protein in neuronal axons that regulates microtubule stability, neurite outgrowth, and other microtubule -dependent functions. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Six tau isoforms are produced in adult Human brain by alternative mRNA splicing from a single gene. Inclusion of a 31 amino acid repeat encoded by exon 10 of the tau gene gives rise to the three isoforms with four microtubule-binding repeats each. The other three isoforms have three repeats each. Abundant neurofibrillary lesions made of the protein constitute a defining neuropathological characteristic of Alzheimer's disease. Filamentous tau protein deposits are also the defining characteristic of other neurodegenerative diseases, many of which are frontotemporal dementias or movement disorders, such as Pick's disease, progressive supranuclear palsy, and corticobasal degeneration.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Alzheimer’s disease brain

Cellular Localization:
Cytoplasmic

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Recombinant protein encoding C-terminal part of human Tau.

Purity:

Serum

Form

Supplied as a liquid in 0.05% sodium azide.

Specificity:

Recognizes human Tau.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Brion et al. Neuroscience 2: 89, 1990. 2. Butner et al. J Cell Biol 115: 717, 1991. 3. Goedert et al. Proc Natl Acad Sci 85: 4051, 1998. 4. Michel et al. Annals of the New York Academy of Sciences 920: 74, 2000. 5. Mukrasch et al. J Biol Chem 282:12230, 2007.