Technical Data
T1030-06T
Tau (Tau Protein, Microtubule-associated Protein, MAP), Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MTBT1, TAU)
Description:
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
The tau/MAP repeat binds to tubulin. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments and straight filaments, mainly composed of hyperphosphorylated forms of Microtubule-associated protein Tau. Defects in Microtubule- associated protein Tau are a cause of frontotemporal dementia and Parkinsonism linked to chromosome 17, as well as a number of other neurodegenerative diseases.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin-embedded/Frozen sections): 1:100-1:1000
Western Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Recombinant human microtubule-associated protein tau (aa 240-450). Cellular Localization: Cytoplasm; cytosol. Cell membrane. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder. Reconstitute with 250ul sterile 40-50% glycerol, ddH2O.
Specificity:
Recognizes human microtubule-associated protein tau. Recognizes both phosphorylated and non-phosphorylated forms of Tau. Species Crossreactivity: rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goedert M, et al. (1988) Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055. 2. Goedert M, et al. (1989) Neuron 3:519-526. 3. Lee G, et al. (1989) Neuron 2:1615-1624. 4. Andreadis A, et al. (1992) Biochemistry 31:10626-10633. 5. Jakes R, et al. (1991) EMBO J. 10:2725-2729. 6. Cripps D, et al. (2006) J. Biol. Chem. 281:10825-10838. 7. Maas T, et al. (2000) J. Biol. Chem. 275:15733-15740. 8. Nacharaju P, et al. (1997) J. Neurochem. 69:1709-1719. 9. Wintjens R, et al. (2001) J. Biol. Chem. 276:25150-25156. 10. Oliva R, et al. (2004) Ann. Neurol. 55:448-449.