Technical Data
Tau (Microtubule-associated Protein Tau, Neurofibrillary Tangle Protein, Paired Helical Filament-tau, PHF-tau, MAPT, MAPTL, MTBT1)
Tau is a collection of microtubule-associated proteins that is involved in microtubule assembly and stabilization. In adult human brain, 6 isoforms ranging between 352 and 441aa in length are produced as a result of alternative RNA splicing. The expression of tau isoforms is developmentally regulated, as only the smallest tau polypeptide is expressed in fetal brain. Hyperphosphorylated Tau is the major component of the paired helical filament of Alzheimer’s Disease. Phosphorylation-dependent anti-Tau antibodies are used to identify specific aa that are phosphorylated in tau from normal brain and Alzheiner’s disease brain. The Tau proteins, esp. in developing brain and in Alzheimer brains, were found to phosphorylate in vivo at many different sites.

Suitable for use in Dot Blot, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Dot Blot: 1:500-1:2000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
25ug-20°CBlue IceHumanRabbit
Synthetic peptide (SPVVSGDT) corresponding to human Tau around Serine 404.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Recognizes human Tau. Species Crossreactivity: mouse at 52-68kD, rat, canine and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Billingsley, M. et al. Biochem J 323, 577 (1997). 2. Geodert, M. et al. EMBO J 8, 393 (1989). 3. Goedert, M. et al. Neuron 3, 519 (1989). 4. Cleveland, D. et al. J Mol Biol 116, 207 (1977).