Technical Data
T1037-01
Tau (Tau Protein, Microtubule-associated Protein, MAP)
Description:
Tau proteins are microtubule associated proteins (MAPs). Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Not suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Native verified): 2ug/mg protein lysate Use Protein G
Western Blot: 1-2ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
IMR5 cells, Human T98G glioblastoma cells or Alzheimer’s disease brain tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG13F348Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceBovineMouse
Concentration:
~0.2mg/ml
Immunogen:
Puri ed bovine microtubule-associated proteins. Epitope: Middle of Tau Cellular Localization: Cytoplasmic: MW of Antigen: 45-68kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T1037-01X.
Specificity:
Recognizes the nonphosphorylated and phosphorylated forms of bovine Tau. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Does not crossreact with other MAPs or tubulin. Species Crossreactivity: human, sheep, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lopresti, P., et al., Proc. Natl. Acad. Sci. USA, 92: 10,369–10,373 (1995).