Technical Data
T1037-01C
Tau (Neurofibrillary Tangles Marker, Microtubule-associated Protein, MAP, Paired helical filament-tau, PHF-tau)
Description:
Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I and II. Type II MAPs include MAP2, MAP4, and tau, and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

Applications:
Suitable for use in Flow Cytometry, Western Blot, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:160
Western Blot: 1:5000-20000
Immunocytochemistry: 1:100-500
Optimal dilutions to be determined by the researcher.

Positive Control:
Human brain tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9i222Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Phospho-synthetic-peptide corresponding to residues surrounding Ser622 of human Tau 37.
Purity:
Supernatant
Form
Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human Tau phospholyated and unphospholyated on Ser622. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.