Technical Data
T2026
TCF-4 (T-cell Transcription Factor 4)
Description:
TCF-4, transcription factor 4, is a basic helix-turn-helix transcription factor. This protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG')-a motif first identified in immunoglobulin enhancers. The gene for TCF-4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described. TCF4 is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

Applications:
Suitable for use in Western Blot, ELISA, Supershift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml. Detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4.
Supershift Assay: Reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA).
Immunoprecipitation: Reported to immunoprecipitate TCF-4 from SW620 whole cell lysates as a complex with b-Catenin.
Immunocytochemistry/Immunohistochemistry: Reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS.
Optimal dilutions to be determined by the researcher.

Positive Control:
DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a0.T.149Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human TCF-4 (aa31-331) (Accession# CAB97213.1)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity:
Recognizes human TCF-4 at ~66kD. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Amir, A.L., et al. (2003). J Biol Chem. 278: 30828-30834. 2. Persad, S., et al. (2001). J Cell Biol. 153: 1161-74. 3. Rockman, S.P., et al. (2001) J Biol Chem. 276: 45113-9. 4. Barker, N., et al., Am. J. Pathol. 154: 29-35 (1999). 5. Korinek, V., et al., Nat. Genet. 19: 379-383 (1998).