Technical Data
T2200-01B
Tec (PSCTK4, Tyrosine-protein kinase Tec)
Description:
Tec is a member of the Tec family of non-receptor protein-tyrosine kinase (PTK) which also includes Btk, emtk/itk/Tsk, Rlk/Txx and Bmx (1). Tec is composed of a pleckstrin domain (PH), an SH3 and SH2 domain, a catalytic kinase domain and a Tec homology domain (TH) (2). Tecs TH domain interacts with the SH3 domain of Grb2, Src family PTKs ( Fyn, Lyn, and Hck), Vav, and c-Kit. Tec is mostly expressed in hematopoietic cells. Tecs SH3 domain interacts with DC28 and has been shown to phosphorylate p62 dok, CD28 substrate (3). Tec can phosphorylate Jak kinase in hematopoietic cells (4) and can also be phosphorylated by Src member Lyn on multiple tyrosine residues (5).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:30
Western Blot: 1:2000-1:5000. Band at ~66kD.
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k392Supernatant
SizeStorageShippingSourceHost
100ul 4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues in the PH domain of human Tec.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.5% BSA, 40% glycerol.
Specificity:
Recognizes human Tec. at ~66kD. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bolen, J. B. (1995) Curr. Opin. Immunol. 7, 306-311 2. Vihinen, M., Nilsson, L., and Smith, C. I. (1994) FEBS Lett. 350, 263-265 3. Wen-Chin Yang, et al J Biol Chem, Vol. 274, Issue 2, 607-617, January 8, 1999 4. Yamashita et al.(1998) Blood 91, 1496-1507 5. H Mano, Y Yamashita, A Miyazato, Y Miura and K Ozawa. 1996, The FASEB Journal, Vol 10, 637-642.