Technical Data
T2399-15B
Telomerase, CT (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)
Description:
Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of the telomerase (1-2). It is also responsible for addition of TTAGGG repeats to the telomere ends of the chromosome, which is important in maintaining cell immortality. (3). In most normal human cells the expression of hTERT is very low. On the other hand, greater than 90 % of human tumors of different pathology and origins have been shown to upregulate telomerase expression (4), making hTERT a cancer-associated target.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100
Western Blot: 1:1,000. Detects a band at ~122kD.
Immunohistochemistry (Frozen): 0.75ug/ml
Immunofluorescence (ICC): 1:50- 1:100.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k393Supernatant
SizeStorageShippingSourceHost
100ul -20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues in the C-terminus of human TERT
Purity:
Supernatant
Form
Supplied as a liquid in PBS, 0.01% sodium azide, 0.05% BSA, 50% glycerol.
Specificity:
Recognizes human TERT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Greider, C.W., and Blackburn, E.H. (1985). Cell 43, 405-413 2. Feng J, Funk WD, Wang SS, et al. Science 1995; 269:1236-41 3.Greider, C.W. (1996). Annu. Rev. Biochem. 65, 337-365 4. Shay JW, Cacchetti SA, Eur J Cancer. 1997:33:787-791