Technical Data
Telomeric Repeat Binding Factor 2, Mouse (TRF2, RAP2) (Control Peptide)
Molecular Biology Storage: -20CShipping: Blue Ice
A 21aa Peptide sequence near the C-terminus of Mouse TRF2. The 21 AA mouse control peptide has no significant sequence homology with TRF1.

The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.

Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2 1122 aa; ~127kD). (1).

Telomeric Repeat binding Factors (TRF1 and TRF2) bind the telomeric TTAGGG repeat. TRFs have been colocalized with telomeric DNA in metaphase cells. TRFs are located at chromosome ends during metaphase. Mouse and human TRF2 are 495 aa and 500 aa, respectively. TRF1 and TRF2 share ~30% homology.
Source: Mouse synthetic peptide
Purity: Highly purified
Concentration: 1mg/ml
Form: Supplied as a liquid in PBS, pH 7.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Meyerson M et al (1997) Cell 90, 785-795; Greenberg RA et al (1998) Oncogene in press; Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review).
2. Chong L et al (1995) Science 270, 1663-1667; Broccoli D et al Gene accession # AF00299 & AF003000.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.