Technical Data
T2550-03
Tenascin (Cytotactin, Tenascin C, Glioma associated Extracellular Matrix Antigen, GMEM, Hexabrachion)
Description:
Tenascin C, also known as J1, is a high molecular weight extracellular matrix glycoprotein that has a complex spatial and temporal pattern of expression during embryogenesis, wound healing, and neoplastic processes.

Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1ug/mL Detection limit ~1ng/well.
Western Blot: 1-2ug/mL Detection limit ~1ng/lane under non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18H168Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanRat
Concentration:
~0.5mg/ml
Immunogen:
NSO-derived rhTenascin C (aa186 -625 ). Accession Number: P24821
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human Tenascin C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.