Technical Data
T2550-23
Tenascin (Cytotactin, Tenascin C, Glioma associated Extracellular Matrix Antigen, GMEM, Hexabrachion)
Description:
Tenascin is a large glycoprotein that forms disulfide-linked hexamers. Tenascin is made up of a series of repeated structural domains including EGF-like repeats, fibronectin type III repeats, and a region homologous to fibrinogen. Splice variants of tenascin differ in their number of fibronectin type III repeats. Tenascin is expressed in distinctive spatial and temporal patterns in most embryonic tissues at sites of tissue remodeling and cell migration. There is little expression of tenascin in adult tissues except, again, at sites of tissue remodeling and cell migration such as surrounding tumors, in healing wounds, and in branching or damaged blood vessels.
Tenascin binds to several cell-surface receptors and mediates cell-substrate adhesion. Nevertheless, tenascin is frequently referred to as an anti-adhesion molecule because for a variety of cell types the presence of tenascin inhibits adhesion to other ECM proteins present. This effect occurs because the binding of tenascin to certain receptors inhibits cell spreading and thereby blocks the stabilization of adhesion that results from cell preading in vitro assays.

Applications:
Suitable for use in Function Blocking, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: Recognizes a 220-240kD band, reduced.
Immunohistochemistry: Frozen and paraffin embedded tissues; citrate treatment useful for paraffin tissues.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after recept. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceChickenRabbit
Concentration:
~1mg/ml
Immunogen:
Purified tenascin from embryonic chicken brain.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS.
Specificity:
Recognizes chicken tenascin. Species Crossreactivity: human and mouse. Under reducing conditions reactivity to chicken is ~100X greater than reactivity with human tenascin. Reactivity with mouse tenascin under reducing conditions is poor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Ja wi ska, A. et al., (2007) Current Biology, 17; 1390-1395. (IH on zebrafish). 1. Feugate JE, et al., BMC cell Biology 3:13 (2002).