Technical Data
Media
Tergitol 7 Agar (Powder)
T2700
Microbiological Grade
Storage RT/4C    Shipping RT
Composition per Liter:
Agar15.0g
Lactose10.0g
Yeast Extract3.0g
Pancreatic Digest of Casein2.5g
Peptic Digest of Animal Tissue2.5g
Tergitol 70.1g
Bromothymol Blue25.0mg
TTC Solution (not included)3.0ml
pH: 6.90.2 at 25C
TTC Solution:
Triphenyl tetrazolium chloride: 1.0g/100ml
Add Triphenyl tetrazolium chloride to ddH2O and bring volume to 100ml. Mix thoroughly and filter sterilize.
Selective medium for Coliform organisms, based on lactose fermentation, from urine and routine water and food testing. Lactose fermenting bacteria appear as yellow colonies. Lactose non-fermenting bacteria appear as blue colonies.

Appearance:
Light green, homogeneous, free flowing powder with soft lumps.

pH (3.3%, 25C):
6.90.2

Formulation (g/L):
Peptic Digest of Animal Tissue 2.5
Pancreatic Digest of Casein 2.5
Yeast Extract 3.0
Lactose 10.0
Tergitol 7 0.1
Bromthymol Blue 0.025
Agar 15.0
Total: 33g/L

Directions per Liter:
Dissolve 33g in 1L of ddH2O stirring gently with heating until completely solubilized. Boil for one minute to dissolve completely. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). After cooling medium to 45-50.C add 3.0 ml of one percent triphenyl tetrazolium chloride, if desired.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


TTC Solution: Triphenyl tetrazolium chloride: 1.0g/100mlAdd Triphenyl tetrazolium chloride to ddH2O and bring volume to 100ml. Mix thoroughly and filter sterilize.

Directions per LiterDissolve 33.125 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Aseptically add 3.0ml of TTC solution. Mix thoroughly. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).StorageStore powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1 Chapman G. H. (1947) J. Bact. 53. 504. 2 Pollard A.L. (1946) Science 103. 758-759. 3 Chapman G.H. (1951) Am. J. Pub. Hlth 41. 1381. 4 Mossel D. A. A. (1962) J. Appl. Bact. 25. 20-29. 5 Kulp W., Mascoli C., Tavshanjian O. (1953) Am. J. Pub. Hlth 43. 1111-1113. 6 E.U. (1998) 98/83/EC of Council of 3rd of November 1998 on the quality of water intended for human consumption. Off. J. Eur. Commun., L3330, 32-54.