Technical Data
Terminal Deoxynucleotidyl Transferase (TdT)
TdT is a nuclear enzyme that catalyzes the random addition of deoxynucleotide triphosphate to the 3-OH groups of single-stranded DNA. A 58kD immunoreactive peptide has been detected in human and mouse lymphoblastoid cell lines. Also, a 58kD-60kD major peptide has been seen on Western Blots of mouse, rat, calf, feline and chicken thymus extracts. Elevated activity of TdT has been shown in neoplasms derived from immature lymphoid cells, lymphoblastic lymphomas (LL), acute lymphoblastic leukemias (ALL), lymphoid blast crisis of chronic myeloid leukemias (CML), and lymphomas. Rabbit anti-TdT may be used to distinguish LL from other lymphomas that do not express TdT.

Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded human tissue sections, or cell smears. Epitope Retrieval Required, use Citrate buffer, pH 6.0.
Enzyme Digestion is not required.
Optimal dilutions to be determined by the researcher.

Positive Control:

Expected Staining Pattern:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
250ul-20CBlue IceRabbit
Purified TdT isolated from calf thymus.
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Recognizes Terminal Deoxynucleotidyl Transferase (TdT).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Orazi, A., et al., Modern Path. 7(5): 582-586 (1994). 2. Orazi, A., et al., AJCP 102(5): 640-645 (1993). 3. Suzumiya, J., et al., J. Pathol. 182: 86-91 (1997). 4. Adriaansen, H., et al., Leukemia 7(3): 472-481 (1993). 5. Urbano-Ispizua, A., et al., British J. Haematology 81: 178-183 (1992).