		Technical Data
T2965-01C	Tetracycline

Description:
Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 20ug/ml
Optimal dilutions to be determined by the researcher.

Match To: T2965-01B

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Sheep

Concentration:

~1mg/ml (U.V. abs)

Immunogen:

Tetracycline(4)-BTG.

Purity:

Purified.

Form

Supplied as a liquid in 20mM PBS, 0.09% sodium azide, pH 7.2.

Specificity:

Cross-Reactivity: Tetracycline 100; Chlortetracycline 3.0; Oxytetracycline 1.0.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.