Technical Data
T2965-02
Tetracycline
Description:
Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability.

Tetracyclines are variably absorbed after oral administration and penetrate into most tissues and body fluids, with the highest levels in kidney and liver. All tetracyclines are excreted in urine and faeces, either as the parent molecule or as microbiologically inactive forms. The inactive 4-epimers (33) are thought to be artefacts produced by chemical rather than biological processes and can form spontaneously during sample preparation (32). Tetracyclines are used extensively in veterinary medicine and their use in food producing animals could result in potentially harmful concentrations in tissue, organs and milk. The potential risk is reduced by withdrawal of the drug for a fixed period before slaughter, although residual levels may remain.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 5.0ug/ml
Optimal dilutions to be determined by the researcher.

Match To: T2965-01A

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabPurified
SizeStorageShippingSourceHost
100ul-20CBlue IceSheep
Concentration:
~1mg/ml (U.V. abs)
Immunogen:
Tetracycline Amide-BTG.
Purity:
Purified.
Form
Supplied as a liquid in 20mM PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes Tetracycline
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.