Technical Data
The tetracyclines are a group of closely related bacteriostatic antibiotics with similar antibacterial spectra. The tetracyclines are variably absorbed after oral administration and penetrate into most tissues and body fluids, with the highest levels in kidney and liver. All tetracyclines are excreted in urine and faeces, either as the parent molecule or as microbiologically inactive forms. The inactive 4-epimers (33) are thought to be artefacts produced by chemical rather than biological processes and can form spontaneously during sample preparation (32). Tetracyclines are used extensively in veterinary medicine and their use in food producing animals could result in potentially harmful concentrations in tissue, organs and milk. The potential risk is reduced by withdrawal of the drug for a fixed period before slaughter, although residual levels may remain.

Cross-reactivity standardized to Tetracycline
Cross-reactant %Cross-reactivity
Tetracycline 100
Chlortetracycline 60
Doxycycline 27
Oxytetracycline 76
4-epi-Tetracycline 95
4-epi-Oxytetracycline 71
4-epi-Chlortetracycline 29
Demeclocycline 50
Minocycline 3
Rolitetracycline 81

Cross-reactivity standardized to Chlortetracycline
Cross-reactant % Cross-reactivity
Chlortetracycline 100
Tetracycline 166
Doxycycline 45
Oxytetracycline 94
4-epi-Tetracycline 130
4-epi-Oxytetracycline 87
4-epi-Chlortetracycline 39
Demeclocycline 80
Methacycline 76
Minocycline 5
Rolitetracycline 130

Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG15j209Affinity Purified
500ug4C Do not freezeBlue IceSheep
Tetracycline Amide-BTG
Purified by Protein A affinity chromatography
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Recognizes Tetracycline and Tetracycline derivatives
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.