The tetracyclines are a group of closely related bacteriostatic antibiotics with similar antibacterial spectra. The tetracyclines are variably absorbed after oral administration and penetrate into most tissues and body fluids, with the highest levels in kidney and liver. All tetracyclines are excreted in urine and faeces, either as the parent molecule or as microbiologically inactive forms. The inactive 4-epimers (33) are thought to be artefacts produced by chemical rather than biological processes and can form spontaneously during sample preparation (32). Tetracyclines are used extensively in veterinary medicine and their use in food producing animals could result in potentially harmful concentrations in tissue, organs and milk. The potential risk is reduced by withdrawal of the drug for a fixed period before slaughter, although residual levels may remain.
Cross-reactivity standardized to Tetracycline
Cross-reactivity standardized to Chlortetracycline
Cross-reactant % Cross-reactivity
Suitable for use in ELISA. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.