Technical Data
Tetracycline Repressor (TetR)
Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability.

The 23kD Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceRabbit
Not determined
Recombinant TetR (tetracycline repressor) produced in E. coli.
Supplied as a liquid in PBS, 50% glycerol.
Recognizes the TetR portion of bait fusion protein in the yeast two-hybrid system. Detects a band of ~37kD by Western blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pook, E., et al., Eur. J. Biochem. 258:915-922 (1998)2. Hillen, W. and Berens, C., Annu. Rev. Microbiol. 48:345-369 (1994)3. Gatz, C., et al., Biospektrum 1:23-29 (1995)4. Mayford, M., et al., Science 274:1678 (19965. Angew Chem Int Ed Engl. 2000 Jun 16;39(12):2042-2052