Technical Data
T2965-35B
Tetracycline Repressor (TetR)
Description:
Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:2000
Western Blot: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to an internal portion, between aa 25-75, of E. coli TetR protein (P04483).
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 30% glycerol.
Specificity:
Recognizes the TetR protein. Used to monitor the regulation of gene expression in mammalian cells. The TetR antibody is not reactive to human or other mammalian proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pook, E., et al., Eur. J. Biochem. 258:915-922 (1998) 2. Hillen, W. and Berens, C., Annu. Rev. Microbiol. 48:345-369 (1994) 3. Gatz, C., et al., Biospektrum 1:23-29 (1995) 4. Mayford, M., et al., Science 274:1678 (1996) 5. Angew Chem Int Ed Engl. 2000 Jun 16;39(12):2042-2052