		Technical Data
T3660-06	TGN38 (Trans Golgi Network 38)

Description:
T3660-06 recognizes a glycoprotein designated TGN38 that is found primarily in the Trans-golgi network, and has been found to be an excellent marker for this cellular organelle. TGN38 is likely to have a role in intracellular transport. It is the homologue of human TGN46.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence (IC). Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:100. Fixation with 3% paraformaldehyde or methanol is recommended
and incubation for 60 minutes at RT in blocking buffer (0.1% gelatin in TBS/triton)
Western Blot: 1:500-1:1000. 5% milk/TBS (+ 0.1% Tween-20) and incubation for 60 minutes at RT
Immunohistochemistry (Paraffin):1:100-1:200. Fixation with methanol or methanol/acetone recommended
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Rat	Sheep

Concentration:

Not Determined

Immunogen:

Recombinant fusion protein corresponding to extracellular domain of TGN38.

Purity:

Serum

Form

Supplied as a liquid, pH 7.2, 1% BSA, 0.09% sodium azide.

Specificity:

Recognizes rat TGN38. TGN38 is a heavily glycosylated protein of around 80-90kD. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.