Technical Data
T5000
Thialysine (S-Aminoethyl-L-cysteine hydrochloride)
1g
5g
5g
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Technical Data |
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T5000 |
Thialysine (S-Aminoethyl-L-cysteine hydrochloride) |
1g 5g |
| Biochemicals | Storage: 4°CShipping: Blue Ice |
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The lysine analog, thialysine, has been implicated in the mechanism for the inhibitory effect of the on human acute leukemia Jurkat T cells. When Jurkat T cells were treated with thialysine (0.32-2.5mM), apoptotic cell death along with several biochemical events such as mitochondrial cytochrome c release, caspase-9 activation, caspase-3 activation, degradation of poly (ADP-ribose) polymerase, and DNA fragmentation was induced in a dose- and time-dependent manner. However, these thialysine-induced apoptotic events were significantly abrogated by an ectopic expression of Bcl-xL, which is known to block mitochondrial cytochrome c release. Decylubiquinone, a mitochondrial permeability transition pore inhibitor, also suppressed thialysine-induced apoptotic events. Comparison of the thialysine-induced alterations in the cell cycle distribution between Jurkat T cells transfected with Bcl-xL gene (J/Bcl-xL) and Jurkat T cells transfected with vector (J/Neo) revealed that the apoptotic cells were mainly derived from the cells accumulated in S and G2/M phases following thialysine treatment. The interruption of cell cycle progression in the presence of thialysine was accompanied by a significant decline in the protein level of cdk4, cdk6, cdc2, cyclin A, cyclin B1, and cyclin E. These results demonstrate that the cytotoxic activity of thialysine toward Jurkat T cells is attributable to not only apoptotic cell death mediated by a mitochondria-dependent death signaling pathway, but also interruption of cell cycle progression by a massive down-regulation in the level of cdks and cyclins. CAS Number: 4099-35-8 Molecular Formula: C5H12N2O2S·HCl Molecular Weight: 200.70 |
Assay by TLC: >99% Appearance: Off-white powder Solubility (H2O, 50mg/ml): Light tan, clear, complete Assay (Elemental Analysis): ~81% based on Nitrogen content Assay (Elemental Analysis): N ~13% Methanol/Chloroform/Ammonia: UV, Chlorine-Tolidine (2/2/1) ~17% (2/2/1) Isopropanol/Ammonia Conc.: 1/1 n-Butanol/Acetic Acid/H2O: 4/2/2 Plate: Silicagel 60 F254 Chloride Content: (Titration) ~17% (AgNO3) Melting Point: ~201°C Specific Rotation (a,24 D): As reported (1% in water) Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Jun, D.Y., et al., Biochem Pharmacol. 66(12): 2291-2300 (2003). Mechanism underlying cytotoxicity of thialysine, lysine analog, toward human acute leukemia Jurkat T cells, Laboratory of Immunobiology, Department of Microbiology, College of Natural Sciences, Kyungpook National University, 702-701 Taegu, South Korea.
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