Technical Data
T5010-02D
Thioredoxin (TRDX, TRX 1, TRX1, TRX-1, TRX, TXN, TXN Protein, ATL-derived Factor, ADF, DKFZp686B1993, MGC61975, Surface-associated Sulphydryl Protein, SASP)
Description:
Thioredoxin (TRX) is a small ubiquitous protein (MW12kDa) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site.This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation.The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19F206Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant human Thioredoxin (TRX).
Purity:
Ascites
Form
As reported
Specificity:
Species Crossreactivity: Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985)
2) Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988)
3) Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996)