Technical Data
Thioredoxin Domain Containing 4 (Thioredoxin Domain-containing Protein 4, TXNDC4, Endoplasmic Reticulum Resident Protein 44, ER Protein 44, ERp44, KIAA0573, PDIA10, UNQ532/PRO1075)
TXNDC4/ERp44/KIAA0573 was identified via its interaction with Erol-Lalpha, which in turn has been shown to regulate oxidative protein folding by selectively oxidizing protein disulfide isomerase. TXNDC4 is induced during ER stress and forms mixed disulfides with both hEROs and cargo folding intermediates. Over expression has been shown to alter the equilibrium of the different Ero1-Lalpha redox isoforms suggesting that TXNDC4 may be involved in the control of oxidative protein folding. It has also been suggested that TXNDC4 senses the environment in the ER lumen because it modulates IP3R1 (1,4,5-triphosphate receptor type 1) activity thereby regulating cytosolic calcium concentrations and intraluminal conditions.

Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 3-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2b,k3C7Affinity Purified
50ug-20CBlue IceHumanMouse
Recombinant corresponding to aa30-407 from human TXNDC4 with GST tag. MW of the GST tag alone is 26kD.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2.
Recognizes human TXNDC4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.