Technical Data
T5010-11J1
TXNDC4 (Endoplasmic Reticulum Resident Protein 44, ER Protein 44, ERp44, Thioredoxin Domain-containing Protein 4, KIAA0573, ERP44, UNQ532/PRO1075)
Description:
Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1L and ERO1LB in the endoplasmic reticulum.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.2-0.6ug/ml, observed in lysates of cell line K562 on ~47kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-EQKDSDNYRVFER, from human TXNDC4, at the internal region of the protein (NP_055866.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human TXNDC4. Species sequence homology: Bovine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anelli T, Ceppi S, Bergamelli L, Cortini M, Masciarelli S, Valetti C, Sitia R. Sequential steps and checkpoints in the early exocytic compartment during secretory IgM biogenesis. EMBO J. 2007 Oct 3;26(19):4177-88. Epub 2007 Sep 6.