Technical Data
T5010-14E
Thioredoxin Reductase, Recombinant, E. coli (TRXR, trxB)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E.coli TRXB protein was expressed in E. coli.

Source:
Recombinant corresponding to aa1-321 of human TRXB 6x His tagged expressed in E. coli.

AA Sequence:
MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG PPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP AAGHQMNGTN QHFRDCNPKH SGGSSTPGGS GGSSTPGGSG SSSGGGAGSS NSGGGSGSGN MPASVAHVPA AMLPPNVIDT DFIDEEVLMS LVIEMGLDRI KELPELWLGQ NEFDFMTDFV CKQQPSRVSC LEHHHHHH

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
34.6kD
Source: E. coli
Purity: ~ 90% (SDS-PAGE). Purified by using conventional chromatography techniques.
Concentration: As reported
Form: Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Faulkner MJ., et al. (2008) Proc Natl Acad Sci U S A. 105(18):6735-40. 2. Takahata M., et al. (2008) J Biochem. 143(4):467-73.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.