Technical Data
T5010-14E
Thioredoxin Reductase, Recombinant, E. coli (TRXR, trxB)
100ug
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Technical Data |
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T5010-14E |
Thioredoxin Reductase, Recombinant, E. coli (TRXR, trxB) |
100ug |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E.coli TRXB protein was expressed in E. coli. Source: Recombinant corresponding to aa1-321 of human TRXB 6x His tagged expressed in E. coli. AA Sequence: MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG PPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP AAGHQMNGTN QHFRDCNPKH SGGSSTPGGS GGSSTPGGSG SSSGGGAGSS NSGGGSGSGN MPASVAHVPA AMLPPNVIDT DFIDEEVLMS LVIEMGLDRI KELPELWLGQ NEFDFMTDFV CKQQPSRVSC LEHHHHHH Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Molecular Weight: 34.6kD |
Source: E. coli Purity: ~ 90% (SDS-PAGE). Purified by using conventional chromatography techniques. Concentration: As reported Form: Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Faulkner MJ., et al. (2008) Proc Natl Acad Sci U S A. 105(18):6735-40. 2. Takahata M., et al. (2008) J Biochem. 143(4):467-73.
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