		Technical Data
T5010-14F	Thioredoxin Reductase 1 (Thioredoxin Reductase 1, Cytoplasmic, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, GRIM-12, Gene Associated with Retinoic and IFN-induced Mortality 12 Protein, KM-102-derived Reductase-like Factor

Description:
The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1,mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena.

Applications:
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000
Immunoprecipitation: 1-2ul/400ul lysates.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysates

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	10K367	Ascites

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Recombinant human protein purified from E.coli.

Purity:

Ascites

Form

Supplied as a liquid in PBS, 50% glycerol.

Specificity:

Recognizes human Thioredoxin Reductase 1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.