Technical Data
T5010-16C
Thioredoxin Reductase 2 (Thioredoxin Reductase 2, Mitochondrial, Selenoprotein Z, SelZ, TR-beta, Thioredoxin Reductase TR3, TXNRD2, KIAA1652, TRXR2)
Description:
The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1,mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena.

Applications:
Suitable for use in Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 1-2ul/400ul lysates
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10K371Ascites
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant human protein purified from E.coli.
Purity:
Ascites
Form
Supplied as a liquid in PBS, 50% glycerol.
Specificity:
Recognizes human Thioredoxin Reductase 2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.