Technical Data
Thioredoxin Reductase 2 (Thioredoxin Reductase 2, Mitochondrial, Selenoprotein Z, SelZ, TR-beta, Thioredoxin Reductase TR3, TXNRD2, KIAA1652, TRXR2)
Thioredoxin Reductase 2 is a 56kD, ubiquitously expressed, mitochondrial selenoprotein and member of the class-I pyridine nucleotide-disulfide oxidoreductase family of proteins. Human TRXR2 is synthesized as a 524aa precursor that contains a 36aa transit peptide and a 488aa mature chain. A selenocysteine residue at position 523 is essential for enzymatic activity. Alternate splicing produces four isoforms. Human TRXR2 shares 86-85aa identity with mouse and rat TRXR2, respectively. TRXR2 maintains thioredoxin in a reduced state and is implicated in the defense against oxidative stress. It may also play a role in redox-regulated cell signaling.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2B11n189Affinity Purified
100ug-20CBlue IceHumanMouse
Not determined
Recombinant corresponding to aa268-393 human Thioredoxin Reductase 2 expressed in E. coli (Q9NNW73).
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Recognizes human Thioredoxin Reductase 2. Does not react with recombinant mouse TRXR2, recombinant human TRXR1, rhTRXR3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.