Technical Data
T5011-01
Thioredoxin (TRX)
Description:
Thioredoxin catalyzes disulfide exchange in proteins via an oxidation/reduction reaction mechanism and is used for apoptosis inhibition in human PBL blasts and Jurkat T-cells. Thioredoxin also functions as a catalyst for filamentous phage assembly and as an accessory protein for phage T7 DNA Polymerase.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:500
Western Blot: 1:50000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19F643Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Protein containing 109aa of TRX 1 from E. coli
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes a lineal epitope of thioredoxin fused to recombinant proteins expressed from pET-32 expression vector.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
LaVallie, E.R. et. al. (1993) Biotechnology 11,187 - 193. Lim, C-J. et. al. (1985) J. Bacteriol. 163,311 - 316.