Technical Data
T5050-12A
Thrombopoietin, Recombinant, Mouse (THPO, TPO, C-mpl Ligand, ML, Megakaryocyte Colony-stimulating Factor, MKCSF, Megakaryocyte Growth and Development Factor, MGDF, MGC163194, MPLLG, Myeloproliferative Leukemia Virus Oncogene Ligand)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
TPO (Thrombopoietin, also known as Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulation levels of platelets in vivo. The murine TPO is a soluble 18.7kD protein containing 174 amino acid residues, which comprises the receptor binding domain of the Mpl-ligand protein.

Source:
Recombinant mouse Thrombopoietin expressed in E. coli.

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells is < 1.0ng/ml, corresponding to a specific activity of > 1x10e6 units/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18.7kD
Source: E. coli
Purity: ~98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug.
Form: Supplied as a lyophilized powder from 10mM Tris, pH 8.0. Reconstitute with sterile ddH20 to a concentration of 0.5-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.