Technical Data
T5100-06AX
Thrombospondin (TSP) (BSA & Azide Free)
Description:
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Applications:
Suitable for use in Western Blot. Not suitable for Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot (Reduced and non-reduced): 0.5-1.0ug/ml for 2 hours at RT.
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13F350 Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human TSP. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T5100-06A.
Specificity:
Recognizes human TSP by at ~450kD (non-reduced) and ~170-180kD (reduced). Displays no crossreactivity with fibronectin, fibrinogen or von Willebrand factor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit VM, et al. J Biol Chem, 1986, 261:1962- 8. 2. Galvin NJ, et al. J Cell Biol, 1987, 104:1413- 22. 3. Dixit VM, et al. Biochemistry, 1985, 24:42705.